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Mock-transfected cells at the wound edge protruded broad lamellipodia and displayed polarity toward the wound (Figure 4B, left, and Movie 1A). Microscopy of mock- or ADAM12-transfected CHO-α4/GFP cells in scratch wound migration assays. (A) CHO-α4/GFP cells, which express both the α4β1 and α5β1 integrins, were either mock transfected or transfected to express full-length ADAM12.
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Go with mobile browser access and home screen shortcut as a safe option. If an iOS application is stated, verify using official brand resources and publisher credentials in store entry. App Store apps can’t be uniformly confirmed through publicly accessible materials. Rely on mobile browser and add home screen shortcut.
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Cells expressing α4β1 but not α5β1 integrin bind to recombinant ADAM12 disintegrin domain. A previous study demonstrated that GFP-tagged α4 integrin behaves like untagged α4 integrin (Pinco et al., 2002). Transwell migration assays were performed as described previously (Liu et al., 1999), with the following modifications.